The Microtainer collection tubes are designed for blood collection transport and centrifugation. The system consists of an unbreakable translucent polypropylene tube with filling guidelines, a flow top collector with a wide mouth that encourages blood flow, and a color-coded stopper for identification of additive and sure seal.
1. A lavender stopper indicates EDTA is the anticoagulant. This tube is suitable for routine hematology procedures.
2. A red stopper indicates that there is no additive. This tube is suitable for laboratory tests requiring serum.
3. A green stopper indicates that lithium heparin is the anticoagulant. This tube is suitable for laboratory tests requiring heparinized plasma.
II. Clinical Significance: N.A.
No special patient preparation is needed. Appropriate puncture sites include the fingertip of an adult or child, or the heel of an infant. Minimum sample volume is 200 µl. Optimum sample volume is 400 µl.
1. Microtainer MAP Microtube for Automated Process with K2EDTA (CardinalHealth #363706)
2. Microtainer Tube with No Additive (CardinalHealth #365957)
3. Microtainer Tube with Lithium Heparin (CardinalHealth #B2975-11A)
4. Gauze sponges (Fisher #22362178)
5. Alcohol [70% Isopropanol] Prep Pads (CardinalHealth #40000-090)
V. Reagents: N.A.
VI. Standardization: N.A.
1. Specimen Collection: Refer to the Capillary Puncture; Finger Using the BD Microtainer Contact-Activated Lancet and Capillary Puncture; Heelstick Using the Tenderfoot Device procedures.
Note: Collect specimen in lavender Microtainer first if several blood samples are to be collected.
A. Cleanse the puncture site with the alcohol prep pad, allow to dry.
B. Pierce the skin using appropriate device Refer to the Capillary Puncture; Finger Using the BD Microtainer Contact-Activated Lancet and Capillary Puncture; Heelstick Using the Tenderfoot Device procedures.
C. Wipe away the first drop of blood with a gauze sponge.
a. For Heparin and No Additive:
Hold the Microtainer with flow top collector vent hole in an upward position (if a vent is present). Touch the collector tip to the underside of the drop of blood. Allow the blood to flow into the collection device and down the tube wall. Avoid scraping the skin surface to collect the blood sample.
b. For EDTA MAP:
Hold the BD MAP at an angle from the surface of the puncture site. Touch the integrated collector end to the drop of blood. After collecting 2-3 drops, the blood will freely flow down the interior tube wall. Fill the BD MAP between 250µl and 500µl to achieve proper blood to additive ratio. A minimum of 375µl is required for automated processing.
D. Fill the heparin and EDTA Microtainer tubes to between the fill marks. The volume colleted must fall within this range or the specimen will be rejected.
E. Fill the “No Additive” tubes to the volume needed for testing.
F. Blood obtained from capillary sites must be free flowing. Never milk the area around the puncture site.
G. When the collection is complete,
a. For Heparin and No Additive:
Remove the collector tip and insert the stopper.
b. For EDTA MAP:
Replace the cap. Push the cap down to completely cover the collar for a secure “snap” fit.
J. Mix the contents of the tubes completely by inverting the tube 8 to 10 times.
K. After collection of the blood specimen is complete, place pressure on the puncture site with a gauze pad until bleeding has stopped. Apply a Band-Aid if needed.
Note: Band-Aids are not recommended for use on small children.
2. Specimen Handling for Microtainer with EDTA:
A. Hematology tubes should be mixed thoroughly just prior to analysis.
B. A minimum of 20 inversions is recommended.
C. Mixing with a mechanical rocker is not advised.
3. Specimen Handling for Microtainer with no additive:
A. Allow the blood to clot for a minimum of 30 minutes.
B. Centrifuge the tube in a Microtainer tube holder for 10 minutes at 1300 rcf.
4. Specimen handling for the Microtainer with Lithium Heparin:
Centrifuge the tube in a Microtainer tube holder for 10 minutes at 1300 rcf.
VIII. Limitations: N.A.
IX. Results Derivation: N.A.
X. Expected Result(s) and/or Critical Values: N.A.
XI. Quality Control:
If blood flow is not adequate to collect minimum volume requirement of 200 µl, a new puncture is performed and a new Microtainer is used. Discard the previous Microtainer.
1. Microtainer Brand tube with EDTA, BD365974, product circular, Becton Dickinson and Company, Rutherford, New Jersey.
2. Microtainer Brand tube with no additive, BD35973, product circular, Becton Dickinson and Company, Rutherford, New Jersey.
3. Microtainer Brand tube with Lithium Heparin, BD35973, product circular, Becton Dickinson and Company, Rutherford, New Jersey.
4. BD Microtainer MAP (Microtube for Automated Process) w/ K2 EDTA package insert. Becton Dickenson Company. Franklin Lakes, NJ. 4/2010.
i. 12-22-88 Steven Hafenbredl
ii. 8-9-92 Jim Powers
iii. March 2003 R. Stoffel (Revised: VII.1.F-G. proper fill; VII.3-4. proper centrifugation.)
iv. March 2011 L. McGovern (Revised: for BD Microtainer MAP tubes)
June 2010 L. Watters (Revised: VII.1.D.)
April 2011 R. Schaefer (Revised: VII.1., 1.B.)