I. Principle: N.A.
II. Clinical Significance: N.A.
Urine collected over a 24 hour period using the "P-Splitter".
Note: The "P-Splitter is used when more than one assay is requested on a single 24 hour urine and the tests require different preservatives.
The specimen must be kept on ice or refrigerated throughout the entire collection period.
Click to open Patient Instructions for printing.
1. P-Splitter (Helena Plastics #5-4010-920-010)
2. Identification label with the patient name and a second identifier, such as date of birth or other identification number.
3. "Preservative" label showing type, concentration and amount of preservative along with the initials of the tech adding the preservative.
Note: Label can be made using a label printer.
4. "Start - Stop Time" label.
Note: Label can be made using a label printer.
5. "Chemical Caution” label
Note: Order “Chemical Caution” labels from the Education Specialist - Cathedral Square.
6. “Chemical Hazard” label used to indicate the particular hazard(s) of the chemical added as a preservative.
Note: Order “Chemical Hazard” labels from the Assistant to the Technical Director.
7. 2000 ml volumetric cylinder
8. 13 x 100 Polystyrene tubes (Cardinal Health #13100PS)
A preservative may be required depending on the constituent(s) being assayed. Refer to the Clinical Laboratory Testing Manual or Mayo Access.
1. Collection container preparation:
A. Label both amber collection containers with the patient's name, a second identifier and the name of the test requested.
Note: Nursing staff collecting 24 hr urine specimens on inpatients label the container.
B. Attach a "Start - Stop Time" label to each amber container.
C. Determine from the test requisition and the appropriate manual if the specimen will require a preservative(s). Fill out and attach a “Preservative” label to each of the two amber containers. Write “none” on the label if no preservative is needed. Attach a "Chemical Caution" label if any preservative has been added and the “Chemical Hazars” label for the specific chemical preservative.
D. Write “Keep refrigerated or on ice” on each container.
2. Collection instructions:
A. If the patient is an outpatient, review the "Urine, 24 Hour Collection Instructions; P-Splitter Collection Container" policy with the patient. Give the patient a copy of the instructions along with the two collection containers, funnel, 2 plugs and a plastic bag.
Note: The collection instructions can be printed from the UCL web page under Specimen Handling.
B. If the patient is an inpatient, send the two collection containers, funnel, 2 plugs and a plastic bag to the nursing unit and instruct nursing service to collect the 24 hour urine.
3. Laboratory Processing:
A. Accession the 24 hour urine specimen.
B. Record the time of collection on the requisition.
C. Mix the specimen in each amber container well. Measure the volume of each container using separate volumetric cylinders. Record the volumes of each container on the requisition; add both volumes and record the sum as the "Total Volume". Show calculations on the requisition.
Note: If there is unequal splitting of the 24 hour urine sample for the 2 collection containers, notify the supervisor and/or pathologist before proceeding.
D. Sample aliquots:
Note: Each container is a separate assay. Follow the appropriate instructions in the following parts a. through f. for each container.
a. Fill two urine specimen containers provided by the reference laboratory with well-mixed aliquots of the specimen.
Note: Use a 13 x 100 polystyrene tube for specimens sent to another UCL site and for tests analyzed at the collection site.
b. Label each container with a patient demographic label. Record the date and time of collection, the total 24 hour urine volume and the test ordered.
c. If the specimen will go to an outside reference laboratory, attach the appropriate accession sticker to the container that will be sent.
d. Send one aliquot and the accompanying requisition copy to the referral laboratory.
e. Save the second aliquot.
Note: The second aliquot may be discarded when the final report is returned from the referral laboratory.
f. Discard the residual specimen from the 24 hour collection container.
VIII. Results Derivation: N.A.
IX. Expected Result(s) and/or Critical Values: N.A.
X. Quality Control: N.A.
1. Helena Plastics 1991/92 catalog
2. "Collection and Processing of a 24 Hour Urine" UCL 1993.
3. 1993 Mayo Test Catalog
i. 4-2-79 S. Raymond
ii. 6-10-93 M. Fuller
iii. 7-13-94 R. Schaefer add chemical warning label
June 2010 A. Cone (no changes)
August 2011 J. Schultz (Revised: IV.2., 5., 6.; VI.1.A-C.; labels updated)